Identifying the Point of No Return: Integrating Single-Cell Transcriptomics and Proteomics to Map Irreversible Fibroblast Commitment in Idiopathic Pulmonary Fibrosis (Stage 1 Registered Report)
Idiopathic pulmonary fibrosis (IPF) is a progressive, fatal lung disease caused by aberrant fibroblast activation and extracellular matrix deposition, with a median survival of 3–5 years. Despite two approved antifibrotic therapies, IPF remains incurable. A critical knowledge gap is the identificati...
Readability and Recoverability of Decohered Information
Under unitary quantum mechanics, decoherence redistributes information into system- environment correlations but does not destroy it. The statement that information remains “in the environment” is operationally incomplete: it may be globally present without being locally readable or actively recover...
Analysis of Unique Genes Reveals Potential Role of Essential Amino Acid Synthesis Pathway in Flavobacterium covae Virulence
Flavobacterium covae is one of the four Flavobacterium species causing columnaris disease among warm-water fish. Flavobacterium covae strain 94-081 is highly virulent in channel catfish (Ictalurus punctatus) compared to F. columnare strain ATCC 49512. This study focused on analyzing the unique genes...
Sleep Fragmentation in Neuronal Aβ42-Expressing Drosophila Replication Study (Stage 1 Registered Report)
Sleep disruption is increasingly recognized as an early feature of Alzheimer’s disease (AD). Recent work reported that neuronal expression of human amyloid beta (Aβ42) in Drosophila melanogaster induces fragmented sleep prior to cognitive decline. This Stage 1 Registered Report proposes a direct rep...
Enhancing Engineered Cell Therapies for CNS Conditions (Final Stage 1 Registered Report)
Cell replacement and supportive cellular therapies for central nervous system (CNS) conditions, including neurodegenerative diseases, traumatic brain injuries, stroke, spinal cord injury, and brain aging, are limited by challenges in transplanted-cell differentiation, survival, integration, and matu...
NeuroSC: Leveraging Pretrained Single-Cell Models for Brain Cell Classification (Stage 1 Registered Report)
The immense cellular diversity of the mammalian brain presents a significant challenge for precise cell type classification, particularly when dealing with the noise and sparsity inherent in single-cell RNA sequencing (scRNA-seq) data. We introduce NeuroSC, a computational framework designed to enha...
Sirt6 Deficiency in Microvascular Mural Cells as a Driver of Paracrine Senescence and Impaired Regeneration in Ischemic Limbs (Stage 1 Registered Report)
Peripheral artery disease (PAD) and its severe form, chronic limb-threatening ischemia (CLTI), affect over 200 million people worldwide and represent a major unmet clinical need. Following revascularization, successful limb tissue recovery requires coordinated angiogenesis and myogenesis, yet existi...
Sleep Fragmentation in Neuronal Aβ42-Expressing Drosophila (Stage 2 Registered Report)
Sleep disruption is an early hallmark of Alzheimer’s disease (AD), and prior research studies show that neuronal expression of human amyloid beta (Aβ42) in Drosophila melanogaster induces fragmented sleep. In this Stage 2 Registered Report, we replicated this phenotype using an automated video-based...
De Novo Protein Binder Design for Advanced Glycation End Products: A Computational Approach to Targeting Age-Related Glycative Damage (Stage 1 Registered Report)
Advanced glycation end products (AGEs) accumulate with age and contribute to cardiovascular disease, neurodegeneration, and diabetic complications through receptor for advanced glycation end products (RAGE) activation and direct protein cross-linking. Despite decades of research, no approved therapi...
Transcriptomic Effects of Cannabis-Derived Therapies in a Drosophila model of ALS
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by motor neuron demise, leading to paralysis and death within approximately 3-5 years of symptom onset. Current FDA-approved therapies offer limited disease modification, highlighting the need for treatments...
Hypocapnia, Autonomic Signatures, and Gamma-Band Reconfiguration During Wim Hof Breathing Method and Cold Immersion: A comparative study between naive and Level 2 experts
Background: The Wim Hof Breathing Method (WHBM), which involves controlled hyperventilation interleaved with breath retention, has been proposed to modulate interoception and autonomic tone; however, neural signatures in trained versus naïve practitioners remain incompletely characterised. Methods:...
Transcriptomic Analysis of A Cannabis-Derived Neuroprotective Therapy in a Zebrafish Model of ALS
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by motor neuron loss, and currently has limited therapeutic options.To address the need for more effective therapies, we evaluated CNR-401, a novel cannabis-based multi-component formulation, in a zebrafish...
How Nasal Cavity Structure Influences Empty Nose Syndrome Severity: A CFD-Based Analysis
Empty nose syndrome (ENS) is an iatrogenic condition that can occur after partial, total turbinectomy, or cauterization of the turbinates. It leads to an altered sensation of nasal airflow, breathing difficulties, sleep disturbances, and other symptoms, such as nasal dryness. Although ENS is often d...
Structural Basis for Differential Affinity and Competitive Binding of DNA Polymerase Peptides to the Mycobacterial β-Clamp
The β-clamp is a potential antibacterial target in mycobacteria and required for the processivity of the high-fidelity replicative polymerase DnaE1 during DNA replication. β-clamp also interacts with ImuB, which plays an important role in assembly of the low-fidelity DnaE2 mutasome. When DnaE1 stall...
The Neuromuscular Nexus: Introducing torque chains as a paradigm-shifting framework for physical, mental, and emotional health
This paper highlights a gap in the scientific understanding of the relationship between movement, muscular tension, breathing, the nervous system, and interoception, proposing a novel framework based on previously undefined neuromuscular ‘torque chains’. These torque chains, categorised as internal...
Successful non-surgical management of chronic hip pain in the context of Otto’s disease by targeted retraining of the internal torque chain and psoas major
Background: Total hip arthroplasty (THA) is highly effective for degenerative joint disease but poses challenges in younger patients due to higher complication and revision rates. This case highlights the importance of exploring non-surgical approaches that optimise the neuromuscular system thorough...
Standing on water: postural behavior while balancing on a stand-up paddle board
Humans can learn to maintain upright posture across a range of scenarios. For instance, when stepping from shore onto a stand-up paddle (SUP) board, humans adapt to a dynamic support surface that can move through both external factors (i.e., imposed forces from water) and self-generated actions (i.e...